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D-Sight for FISH


dsight fish
  • High-quality scanning sensor with highly sensitive monochrome camera
  • Integrated 1D/2D barcode reader
  • Digital microscope for real-time live slide viewing
  • Remote digital microscope
  • Optional Images Analysis modules
  • Web application
 

Features

Features

D-Sight for FISH is an automated fluorescence ​digital ​slide ​microscope and ​scanner that allows fast and reliable acquisition, navigation, analysis, archiving and sharing of high quality digital images. Entire slides can be scanned at selectable magnifications with high focus precision and excellent image quality.  D-Sight's dedicated Web application provides unlimited remote access to acquired images and password-protected access to individual files and working environments.
  • 8-slide capacity
  • Motorised revolver with up to 7 high-resolution objectives 
  • Up to 6 fluorescent filters
  • Various hardware profiles (H&E, IHC, haematology, cytology)
  • Two scanning speeds and Z-stacking
  • Selectable magnifications
  • Measuring, mark-up and text tools
  • Analysis modules for FISH amplification, FISH translocation, FISH Spot counting and immunofluorescence
  • Optional enjoy ambidextrous joystick for navigating images
  • Optional macro section slide tray

Fluorescent analysis modules

Fluorescent analysis modules

Gold standard fluorescent in situ hybridisation (FISH) is a valuable research and diagnostics technique; however, it requires the scientist to manually assess each slide using a fluorescent microscope, a procedure that can be laborious and time consuming. D-Sight automated scanners enable high quality image capture and consistent and accurate analysis of FISH slides, minimising user interaction for a faster and more efficient workflow.

  • Automatic previewing, barcode reading and scanning
  • Suspension and tissue FISH analysis
  • Brightfield and fluorescence image acquisition with the same machine (D-Sight FLUO)
  • H&E/FISH Tissue Matching (D-Sight FLUO)
  • Automated capture, segmentation and cell classification
  • Automated and user-defined nuclei segmentation and spot thresholding.
  • Intuitive probe definition
  • Interactive gallery of identified nuclei
  • Darkfield preview for easy localisation of fluorescent samples

 

FISH Amplification module

fish

  • Gene amplification detection
  • Evaluation of the HER2/CEP17 ratio and FGFR1 amplification ratio
  • Manual and automated HER2/neu tissue samples analysis
  • Automated segmentation of clustered and touching cells
  • Detection and enumeration of all signals

 

 

FISH Translocation module

The D-Sight systems with the FISH Translocations analysis module allows fluorescence analysis of one gene rearrangements using a 60x oil immersion objective (or others with lower magnification, if set by the user). The system captures multiple images at a different focal level for each channel and is able to create an automated merged image where the auto-fluorescence effects on the probe channels are completely removed.
  • Identification and evaluation of gene rearrangement
  • Analysis of break-apart or dual fusion probes (ALK, ROS)
  • Automated, computer-aided cell segmentation, signal detection and cell classification
  • 3D distance evaluation using separate Z-stack acquisition for each probe

 

FISH Spot counting module
fish 2

  • Automated cell segmentation, spot signals count and cell classification
  • Statistical analysis of all counted cells
  • User-defined cell clusters based on signal count or amplification ranges

System generate a resume table for each analysed slides were are displayed the total nuclei number, the number of positive and negative number, the nuclei’s number of each positive or negative classes, the test results. As for the HER-2 test the bright field slide can be superimposed to the fluorescent image. FISH amplification is CE IVD certified for HER2 FISH testing when using Vysis PathVysion HER2 DNA Dual Colour Probe Kit. Other  applications are for research use only Immunofluorescence slide scanning

 

Immunofluorescence Slides Acquisition

The D-Sight FLUO/FISH allows defining customised test for immunofluorescence slides acquisition. The test can include several probes each one associated to a specific filter. The system, starting from the image preview, identifies the area to be scanned and digitalise the slide. During the scanning the system captures multiple images at a different focal level for each channel and is able to create an automated merged image where the auto-fluorescence effects on the probe channels are completely removed. If several probes have been used, the image is reconstructed by using different colour the user can choose. 
  • Kidney biopsy by using single probe with FITC filter
  • Skinny biopsy by using single probe with FITC filter
  • Acquisition with up 6 different probes

Web server interface (NAVIWEB)

Web server interface (NAVIWEB)

naviweb 3

naviweb

The NAVIWEB web-based application makes NAVIGO/D-Sight the ideal solution for telepathology, providing access to digitised slides from anywhere with immediate image display and navigation. Once the acquired images are uploaded to a web-server, they can be accessed at any time from any web-device. Different user with access rule and permission can be created. Each scanned slide can be loaded (automatically) in a web server and will be available for navigation. The internet browser can be used to view images with a series of interactive tools. NAVIWEB has an online archive where upload digital images generated by one or more systems NAVIGO/D-Sight connected to the server machine, and through a web interface, it allows the navigation of the slides. NAVIWEB is a solution of remote working for the pathologist, to view scanned images, create work projects, provide and receive telepathology services.

  • No software to download or install
  • Seamless multi-user sharing of digital slidesnaviweb 2
  • Fast image streaming and instant notification to remote users when a case is assigned for
  • Review or real-time consultation
  • Simultaneous examination of sample images and synchronised navigation
  • Easy navigation with the image viewer

Downloads

Downloads

Smart Digital Pathology Brochure

Technical Specifications

Technical Specifications

Reference 48283
Slides 8 slides automatic
Scanning speed 25s per z-stack field for Her-2 FISH at 60x Oil. Few minutes for several hundred cells in FISH analysis at 60x oil immersion
Camera Monochrome
Objective 4x, 10x, 20x, 40x, 60x oil immersion
Number of objectives Up to 7
Scanning magnification 10x, 20x, 40x, 60x
Resolution at 20x 0.5 µm/pixel
Resolution at 40x 0.25 µm/pixel
Resolution at 60x 0.107µm/pixel
Image compression Jpeg2000
Compression level (brightfield) 1x1cm a 20x ≈ 80MB (not compressed ≈ 1GB)
1x1cm a 40x ≈ 140MB (not compressed ≈ 4GB).
Compression level (fluorescence) 1x1mm at 60x ≈ 50MB (not compressed ≈ 300MB)
Illumination Mercury lamp
Barcode reader Yes
Maximum number of filters Up to 6
Digital microscope acquisition Yes
Optional accessories Monitor 24” Full HD, Enjoy joystick
Image analysis modules IHC Analysis module, IHC Membrane, IHC Nuclei, TMA, FISH Amplification, FISH Translocations,Spot Counting
LIS connection Yes
Web application Optional (NAVIWEB)

 Environmental Conditions - Working Session

Temperature 10-40° C
Relative Humidity 8-75% RH (no condensate)

 Electrical Specifications

Power Source AC 100-240V 50/60 Hz

Size

Microscope Unit (HxWxL) 60 x 60 x 40 cm
Processing Unit (HxWxL) 46 x 52 x 21 cm
Display Unit 24" (HxWxL) 46 x 25 x 57 cm

Weight

Microscope Unit 30kg
Processing Unit 15kg
Display Unit 10kg